Low-Cost Smart Culture Chamber for Trichoderma

Idea 1: Bioreactor for submerged liquid fermentation of Trichoderma

What is it?

My first final project idea is to develop a bioreactor for the submerged liquid fermentation of a very important antagonistic fungus in the biological control of phytopathogens. This prototype is a stirred tank bioreactor (STR) specifically designed for the submerged liquid fermentation of the fungus Trichoderma, manufactured using 3D printing and laser cutting technologies.

Its fundamental components include a top motor with internal paddles for homogenization and oxygenation of the medium, automatic pH and temperature controllers with an LCD screen for real-time monitoring, an LED lighting system to regulate the photoperiod, a gas outlet, and a bottom discharge valve for sterile harvesting of the product.

Who will use it?

The equipment will be used primarily by biotechnology researchers and local producers of agricultural bio-inputs who require an efficient technological tool to scale up their fungal cultures under controlled environmental conditions.

Importance

The importance of this prototype lies in its ability to modernize and standardize the production of agricultural bio-inputs, which is vital for the preservation of sensitive ecosystems. By replacing synthetic agrochemicals with this natural preventative, the biodiversity of the soil and water sources of the region are protected, promoting sustainable agriculture that safeguards the health of the Amazon biome.

Idea 2: Low-Cost Smart Culture Chamber for Trichoderma

What is it?

This prototype is a Low-Cost Smart Culture Chamber, specifically designed as an incubator and culture storage facility for the initial phase of isolating and propagating the Trichoderma fungus in Petri dishes.

The chamber integrates sensors to maintain a stable temperature between 25 and 30 degrees Celsius, as well as a humidity control system and LED panels to manage the photoperiod necessary for sporulation. Its modular structure includes a blowhole fan for air circulation, an LCD screen for monitoring, and internal trays, all designed for local assembly with readily available materials.

Who will use it?

The equipment has been designed to be highly replicable, allowing not only the staff of the CITE Productivo Madre de Dios to access cutting-edge technology, but also enabling its implementation by producer associations and small local laboratories.

By using readily available electronic components and easily manufactured structures, access to biotechnological tools is democratized, allowing more people to manage their own culture collections and ensure the propagation of high-quality inoculants autonomously and economically.

Importance

The importance of this incubator lies in its potential to transform regional agriculture, guaranteeing the viability of pure strains that drastically reduce the use of chemical pesticides in the Amazon. By facilitating the local production of an efficient biocontrol agent like Trichoderma, a sustainable alternative is offered that protects the biodiversity of the Amazon biome and the health of the soils.